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Additional info for Woodworking Shopnotes 042 - Stacking Sawhorses
Four principle ions, m/z 477, 473, 381, and 377, are detected and further fragmented by collision with argon-producing daughter ions consistent with derivatives of enterodiol (mw = 302 from m/z 477 and 381 fragments) and enterolactone (mw = 297 from m/z 473 and 377), respectively. The mass loss suggests that m/z 477 and 473 are the glucuronides and m/z 381 and 377 are the monosulfates of enterodiol and enterolactone, respectively. While ESI is probably the most widely used soft ionization technique, APCI can also be used to quantitate lignan metabolites.
In the scan mode, ions entering the mass spectrometer are analyzed over an m/z range from 50 to 800. , 1998). Measurement of the isoflavonoids and their metabolites in urine from those consuming a heavy soy diet can be carried out by HPLC-UV analysis (Franke and Custer, 1994) as well as HPLC-MS. In the latter method, multiple reaction ion monitoring (MRM) is used, combining each parent molecular ion with a specific daughter ion produced by collision-induced dissociation. 0001). However, the concentrations of daidzein detected by the HPLC-HN-APCI-MRM method are 40% lower than those by HPLC-UV.
1989) TSP-MS-MS proved to be a useful technique, yielding valuable structural information absent from the mass spectra of the phytoestrogens recorded under TSP-MS conditions. The resultant product ion spectra contained fragments characteristics of each phytoestrogen subclass, allowing the use of tandem MS to confirm identification and propose structures for unknown compounds. TSPMS-MS analysis of these substances has also been carried out in the neutral loss operating mode by monitoring the neutral loss of ion (due to consecutive releases of CO), common to all members of this family.