By Alan L. Harvey
The concepts of tissue tradition have been brought first and foremost of this century. they've got turn into an increasing number of renowned because it is discovered that they're no longer as tough or as esoteric as a few early protagonists cherished to take care of. many of the paintings played with tradition equipment has easily involved telephone progress and survival. Biologists have lengthy used tradition techniques to supply an easy method within which to review mobile department and multiplication. Any pharmacology performed on cultured tissue used to be principally toxicological or as a part of a screening programme for poten tial anti-cancer medicines. within the final decade there was a superb raise within the use of excitable cells in tissue tradition. Nerves and muscle groups from a large choice of resources can keep their hugely differentiated houses in tradition. Such cultures provide an enticing education to be used in physiological and pharmacological investigations. for this reason, an unlimited volume of labor has been produced, and this booklet is an try to evaluation it. it really is was hoping that it will introduce physiologists and pharmacologists to the possibility of tradition tools for his or her experiments and likewise illustrate to extra conventional tissue tradition clients extra attainable parts of curiosity. through being extra complete in scope and via attempting to focus mostly on drug activities, i am hoping that the current quantity usefully extends the therapy of the topic all started previous within the very good works through Crain (1976) and Nelson and Lieberman (1981).
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Additional info for The Pharmacology of Nerve and Muscle in Tissue Culture
1981); this obviously complicates the determination of a single reversal potential. In addition, pre-synaptic stimulation by glutamate may lead to transmitter release and the added post-synaptic responses may alter the apparent reversal potential (Brookes, 1978). Although glutamate and aspartate can be readily shown to depolarize spinal cord cells in tissue culture, the fundamental properties of the receptors have not yet been characterized. The limiting slope of the log response against log concentration graph for glutamate is close to one, suggesting that one molecule of glutamate is needed to activate each receptor (Barker and Ransom, 1978a).
B. KCI recording electrode. Reversal potential of glycine response similar to that of GABA (-20 mV). Glycine pulse: 16 nA. 100 ms; GABA pulse: 24 nA. 100 ms. Lower calibrations apply to B. From Barker and Ransom (1978a). with permission. 46 Nerve Cells in Primary Culture concentra tion about 100 J,LM (Dichter, 1980). , 1982). Dose response curves for glycine (Dichter, ! 980) have similar slopes to those for GABA. Since responses to GABA, glycine and (3-alanine seem to be mediated by similar changes in C1" permeability, it is feasible that all three amino acids act on the same receptor site.
From Barker and Ransom (1978a). with permission. 46 Nerve Cells in Primary Culture concentra tion about 100 J,LM (Dichter, 1980). , 1982). Dose response curves for glycine (Dichter, ! 980) have similar slopes to those for GABA. Since responses to GABA, glycine and (3-alanine seem to be mediated by similar changes in C1" permeability, it is feasible that all three amino acids act on the same receptor site. However, there are several pieces of evidence against this. g. , 1977); and thirdly, as discussed in detail below, certain drugs can block responses to GABA or to glycine selectively.