Download The Epidermis by William Montagna and Walter C. Lobitz (Eds.) PDF

By William Montagna and Walter C. Lobitz (Eds.)

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9-day embryo 1 2 3 4 5 7 14 26 5 6 18 4 13 2 8 2 4 3 3 7 1 10-day embryo 1 4 7 14 a b 1 2 3 2 1 2 3 1 Feather-like structures. Slow-growing cultures. 6. Primary Dissociated Epidermis and Primary Dissociated Cultured Separately in Diffusion Chambers on the CAM Mesoderm Primary dissociated epidermis would not form a compact mass in the diffusion chamber but spread over the surface of the membrane filter which made u p the floor of the chamber. N o structures were observed to develop, and no keratin was detected in 7 cases.

8. Primary Dissociated Whole Skin Grown on Plasma Clot Feather-like elements arranged in a fabric of skin developed in pellets of dissociated whole skin cells placed in plasma clot (Fig. 14). These resemble the structures which arose in grafts of cells grown for 3 days in culture (Fig. 10). T h e y consisted of rings of keratinizing cells disposed around a lumen which was sometimes empty and sometimes filled with keratin. T h e large number of regularly spaced structures which developed in each graft was notable but all of them were imperfect, lacking barb and barbule cells, barb ridges, and dermal papillae.

5. Feather-like structure which developed from primary dissociated 9-day epidermis (ordinary light). Deeply stained areas are keratinized. feathers which formed from cells of whole skin. In fact, the "keratin a g e " of the feather-like structures was about 1 to 2 days in advance of normal feathers. 3. Aggregates of Dissociated Primary Mesoderm Most grafts failed to form structures which had any resemblance to feathers. I n several grafts, however, it appeared that some rudimentary or- 44 E U G E N E BELL, MARGERY S C H Ü L E R , AND CHARLOTTE MERRILL ganization took place.

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