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EXTRACTION, SEPARATION, & ISOLATION 41 Specific absorption coeflBcients serve for the identification and estima­ tion of the common chlorophylls ( J 5 0 ) . They do not provide a reliable basis for the identification or estimation of substances having the same characteristic spectral absorption curves. With preparations of high purity the spectral absorption curves and the specific absorption co­ efficients should agree with those reported for the individual pigments (see Figs. 4 - 8 and Tables I I I - V ) .

Alternatively, small portions of the plant material may be ground in a mortar with the solvent and sand and filtered or centri­ fuged. Even in a blender, some fresh plant materials, such as the thick rubbery brown algae, will not yield their pigments completely to the organic solvents. Other plant materials do not liberate the pigments with­ out change or alteration of the chlorophylls. With many of these ma­ terials, it is desirable to place the samples in a large volume of boiling water for a minute.

In alkaline solutions (as in the phase test) the chlorophylls are hydrolyzed to green chlorophyllins ( 1 0 2 ) . These reaction products result from splitting of the cyclopentanone ring with the formation of additional carboxyl groups. Their spectra resemble those of the chlorophylls. 8. ALLOMERIZED CHLOROPHYLLS When dissolved in absolute methanol and exposed to air, the chloro­ phylls are oxidized rapidly ( 5 7 ) . In addition to the oxidized chlorophylls that are formed, the reaction mixture yields various methoxy-containing green pigments called allomerized chlorophylls.

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