Download Protein - DNA Interactions by Abelson J.N., Simon M.I., Sauer R.T. PDF

By Abelson J.N., Simon M.I., Sauer R.T.

Tools familiar within the examine of DNA binding proteins are awarded during this quantity. those comprise purification and protein characterization, assays of protein-DNA binding and protein-induced bending, and biochemical and genetic tools for probing the constitution, strength, and specificity of protein-DNA interactions.

Show description

Read Online or Download Protein - DNA Interactions PDF

Similar biotechnology books

Easy Mathematics for Biologists

Good written advisor to the fundamentals akin to concentrations, dilutions, logs and so on. with plenty of worthwhile labored examples. the one challenge is the booklet is a section too simple for all mathematic wishes in the course of a organic measure.

Genetic Modification of Plants: Agriculture, Horticulture and Forestry

Today´s agriculture faces new and hard demanding situations. With glossy equipment greatly utilized, it truly is quite often believed that genetic crop development is a key for expanding yields. even with the dialogue concerning the security of genetically changed (GM) vegetation that continues to be raging within the eu Union the place the take-up of the recent expertise is extra constrained, the cultivation of GM vegetation and plants has exploded around the world.

Biotechnology and Polymers

The time period biotechnology has emerged at the modern scene really lately, however the uncomplicated notion of using usual fabrics, both without delay or in changed types, dates again to antiquity. If we seek the traditional literature, corresponding to the Bible, we discover hundreds and hundreds of examples in which humans hired, or converted, usual fabrics for a number of vital makes use of.

Carbohydrate Biotechnology Protocols

We're in a section of the evolution of biotechnology within which the real and power advertisement value of carbohydrates is changing into appre- ated extra totally. growth in supplying demanding proof to set up the economic worth ofpolysaccharides and oligosaccharides is restricted, as continuously, by means of loss of investment and through a relative scarcity of expert practitioners within the construction and research of these fabrics.

Additional resources for Protein - DNA Interactions

Example text

Heat at 65 ° for 15 min to inactivate the kinase. Let the sample cool to room temperature, and then add 750 /zl ethanol. Mix by inversion. Spin (microcentrifuge) for 15 min at room temperature to pellet DNA. Discard supernatant. 4. Add 225/xl TE. Dissolve the pellet by vortexing. Then add 250/zl phenol-chloroform (1 : 1, v/v). Vortex for 1 min. Spin 5 min to separate phases. Transfer the upper layer to a new tube. Then add 250/zl chloroform-isoamyl alcohol (24 : 1, v/v). Vortex for 1 min. Spin 5 min to separate phases.

48, 507 (1987). e e j. -X. Hofmann, T. Laroche, A. H. Brand, and S. M. ) 57, 725 (1989). g A. R. Buchman, N. F. Lue, and R. D. Kornberg, Mol. Cell. Biol. 8, 5086 (1988). gg K. S. Sweder, P. R. Rhode, and J. L. Campbell, J. Biol. Chem. 263, 17270 (1988). hh M. Cai and R. W. Davis, Mol. Cell. Biol. 9, 2544 (1989). 14 PURIFICATION AND CHARACTERIZATION [2] the preparation of partially purified protein fractions that are suitable for affinity chromatography. General Approach to Purification of Sequence-Specific DNA-Binding Proteins A strategy for the purification of sequence-specific DNA-binding proteins is as follows.

147, 11 (1981). 10 j. Greenblatt and J. ) 24, 421 (1981). 1i M. Sopta, R. W. Carthrew, and J. Greenblatt, J. Biol. Chem. 260, 10353 (1985). 26 PURIFICATION AND CHARACTERIZATION [3] In addition, the commercial availability allows good reproducibility and convenience. Preparation of Protein Ligand Ideally, the protein ligand that is used for affinity chromatography should be pure in order to ensure that every interacting protein that is detected is binding to the ligand rather than to a contaminant in the preparation.

Download PDF sample

Rated 4.52 of 5 – based on 11 votes