Download Practical Methods for Biocatalysis and Biotransformations by John Whittall, Peter W. Sutton PDF

By John Whittall, Peter W. Sutton

Biocatalysts are more and more utilized by chemists engaged in high quality chemical synthesis inside either and academia. this present day, there exists a massive number of high-tech enzymes and full telephone biocatalysts, which upload greatly to the repertoire of man-made chances.

Practical equipment for Biocatalysis and Biotransformations is a “how-to” consultant focussing on commercially to be had enzymes and lines of microorganisms which are without problems received from tradition collections. The resource of beginning fabrics and reagents, tricks, tips and safeguard suggestion (where acceptable) are given to make sure, so far as attainable, that the strategies are reproducible. Comparisons to replacement method are given and proper references to the first literature are stated. Contents include:

  • Introduction to the appliance of biocatalysts within the pharma industry
  • introduction to molecular organic strategies for biocatalyst development
  • Lipase and protease equipment together with enzyme helps and dynamic kinetic resolutions
  • Nitrile hydrolases and hydratases
  • Alcohol dehydrogenases and co-factor recycling
  • Epoxide hydrolases
  • Amino acid synthesis
  • Whole mobilephone processes
  • Other biocatalysts, together with monoamineoxidases, aminotransterases, and lyases

Practical tools for Biocatalysis and Biotransformations is an important textual content providing proven biocatalyst equipment for researchers and postgraduate scholars in and academia.

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Extra resources for Practical Methods for Biocatalysis and Biotransformations

Example text

There are, in addition, a large number of glycosidases which hydrolyse glycosidic bonds. Specificity for 18 Biotransformations in Small-molecule Pharmaceutical Development these enzymes tends to be significantly lower than for the glycosyltransferases, potentially allowing for the development of a more general biocatalyst for glycosylation using the reverse reaction. Glycosides may be accessed through glycosidase-catalysed reverse hydrolysis under thermodynamic or kinetic control by using free or activated sugar donors respectively.

6). 12 Two-enzyme route to 7-ACA A single-step process using one biocatalyst might be expected to provide even greater environmental and cost benefits. In addition to providing a simplified process, the singleenzyme process affords D- -aminoadipic acid as a co-product. Being an optically pure chiral product, -aminoadipic acid is of potential commercial value in contrast to the ammonia and glutamic acid co-products resulting from the two-enzyme process. 3 Application of Biocatalysis in the Pharmaceutical Industry 21 this option proved to be somewhat elusive until the discovery of a cephalosporin C amidase from Pseudomonas sp.

44 Many pharmacologically active glycosides of low or intermediate molecular weight are also produced as natural products, extracted from plants (digoxin), animals (heparin fractions) or microbial cultures (macrolide and aminoglycoside antibiotics). Currently, biocatalytic glycosylation is confined to relatively simple operations rather than in vitro synthesis of these more complex molecules, and natural products may be used as starting materials for semisynthetic molecules. Compared with proteins and nucleic acids, which are produced by template-driven biosynthesis, there is no equivalent to ensure the structural fidelity of carbohydrate macromolecules and other glycosylated products.

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