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Extra info for Pesticide Residues in Food: 2001 Evaluations Part 2 Toxicological (Pt. 2)
All the studies were considered adequate for their intended purpose unless specifically stated in the text. Studies that contained statements of compliance with good laboratory practice (GLP) or had been subjected to quality assurance (QA) audits are identified in the reference list. Diflubenzuron has been known under a number of synonyms and codes, including DU112307, TH6040, Vigilante and Dimilin. The manufacturing process for diflubenzuron is reported to have been consistent over time (Dykstra, 2001), and the results obtained in the 1970s were considered applicable to the technical-grade material produced currently.
All animals were examined in detail weekly, including palpation for tissue masses. Body weights were estimated before dosing and weekly thereafter, and food consumption was estimated weekly. A functional observational battery (FOB) test was performed 1 week before administration of the test materials, at the time of peak effect after administration of the test materials (9-11 h for diazinon, 1 h for triadimefon) and 7 and 14 days after administration (of diazinon) on the 10 animals intended for neurological testing.
5,150,300 or 600 mg/kg bw. Clinical observations were made immediately before blood sampling from the orbital plexus for determination of serum and erythrocyte cholinesterase activity. Five animals of each sex were killed at 3,5 and 9 h and the remainder at 24 h to obtain brains and spinal cord for determination of cholinesterase activity, the samples being stored at -70 to -90 °C. Acetylcholinesterase activity was measured in the cerebellum, cerebral cortex, striatum and hippocampus and in the thoracic spinal cord by a modification of the method of Ellman et al.