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A guide on the production of algal culture for use in shrimp hatcheries. National Prawn Fry Production and Research Centre, Pulau Sayak, Kedah, Department of Fisheries, Ministry of Agriculture, Malaysia. 23pp. W. R. 1967. A practical manual on the use of the Coulter counter in marine research. Coulter Electronics Sales Company, Ontario, Canada, 65 pp. M. R. 1993a. Intensive algae culture techniques. In: CRC Handbook of mariculture. Volume 1. Crustacean Aquaculture, 2nd Edition. P. ). , Boca Raton, Florida, USA, pp 3-13.

1. Introduction Although Brachionus plicatilis was first identified as a pest in the pond culture of eels in the fifties and sixties, Japanese researchers soon realized that this rotifer could be used as a suitable live food organism for the early larval stages of marine fish. The successful use of rotifers in the commercial hatchery operations of the red sea bream (Pagrus major) encouraged investigations in the development of mass culture techniques of rotifers. Twenty five years after the first use of rotifers in larviculture feeding several culture techniques for the intensive production of rotifers are being applied worldwide.

Although the mechanism is not completely understood, it is generally believed that the production of resting eggs is a survival strategy of the population through unfavourable environmental conditions such as drought or cold. 4. Strain differences Only a few rotifer species belonging to the genus Brachionus are used in aquaculture. As outlined in the introduction the most widely used species is Brachionus plicatilis, a cosmopolitan inhabitant of inland saline and coastal brackish waters. It has a lorica 52 length of 100 to 340 µm, with the lorica ending with 6 occipital spines (Fukusho, 1989).

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