Download Genetic Modification of Plants: Agriculture, Horticulture by John J. Finer (auth.), Frank Kempken, Christian Jung (eds.) PDF

By John J. Finer (auth.), Frank Kempken, Christian Jung (eds.)

Today´s agriculture faces new and hard demanding situations. With smooth tools broadly utilized, it's as a rule believed that genetic crop development is a key for expanding yields. inspite of the dialogue concerning the safeguard of genetically transformed (GM) plants that continues to be raging within the eu Union the place the take-up of the recent know-how is extra constrained, the cultivation of GM vegetation and vegetation has exploded everywhere in the world.

Conceived with the purpose of sorting truth from fiction over GM plants, this quantity brings jointly the information of 30 experts within the box of transgenic crops. It covers the iteration and detection of those crops in addition to the genetic features conferred on transgenic crops. furthermore, the booklet appears to be like at a large choice of plants, decorative crops and tree species which are topic to genetic alterations, assessing the dangers enthusiastic about genetic amendment in addition to the aptitude financial merits of the know-how in particular situations. The book’s constitution, with totally cross-referenced chapters, offers readers a short entry to express themes, no matter if that's unique and accomplished info on specific species of ornamentals, or assurance of the socioeconomic implications of GM know-how. With an expanding call for for bioenergy, and the mandatory better yields hoping on wider genetic edition, this booklet offers all of the technical info required to maneuver ahead to a brand new period in agriculture.

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Extra resources for Genetic Modification of Plants: Agriculture, Horticulture and Forestry

Example text

It is not clear whether the pollen tube is actually hollow. It is also unclear whether any DNA is able to enter the ovule. The timing of the cutting of the pollen tube and subsequent DNA introduction must be very precise, to have the DNA enter the cell with the pollen nucleus. The reported efficiency of the process is inexplicably high, considering that <1% of cells that contain DNA introduced via particle bombardment are able to integrate that DNA into their genome. Extensive analysis of soybean plants obtained through the pollen tube pathway suggests that this method is not reproducible (Shou et al.

1). So far a given plants ability to regenerate from fully differentiated tissue is the biggest obstacle for applying the plastid transformation to a large number of plant species. Tobacco is by far the best analyzed system regarding plastid transformation, and therefore most experiments referred to in this section are made in tobacco. 1 Vector Design Flanking Regions Agrobacterium-mediated transformation utilizes universal vector systems (Lee and Gelvin 2008; see also Chapter 1) in which the transgene expression cassette is flanked by two rather short sequence stretches, termed left border (LB) and right border (RB).

Proc Natl Acad Sci USA 82:5824–5828 Hadi MZ, MD McMullen, JJ Finer (1996) Transformation of 12 different plasmids into soybean via particle bombardment. Plant Cell Rep 15:500–505 Hansen G, Das A, Chilton MD (1994) Constitutive expression of the virulence genes improves the efficiency of plant transformation by Agrobacterium. Proc Natl Acad Sci USA 91:7603–7607 Hernandez-Garcia CM, Martinelli AP, Bouchard RA, Finer JJ (2009) A soybean (Glycine max) polyubiquitin promoter gives strong constitutive expression in transgenic soybean.

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