Download Downstream Processing of Proteins: Methods and Protocols by Mohamed A. Desai PDF

By Mohamed A. Desai

Huge time and effort is allotted to introducing mobile tradition and fermentation expertise to undergraduate scholars in academia, normally via a number classes in commercial biotechnology and similar disciplines. equally, a number of textbooks can be found to explain the appli- tions of those applied sciences in undefined. notwithstanding, there was a normal loss of appreciation of the numerous advancements in downstream processing and isolation expertise, the necessity for that's principally pushed through the stringent re- latory specifications for purity and caliber of injectable biopharmaceuticals. this is often quite mirrored by way of the overall absence of insurance of this s- ject in lots of biotechnology and comparable classes in academic associations. For a substantial whereas i've got felt that there's expanding want for an introductory textual content to varied features of downstream processing, fairly with appreciate to the wishes of the biopharmaceutical and biotechnology ind- attempt. even though there are lots of texts that hide quite a few features of protein purification strategies in isolation, there's a desire for a piece that covers the large diversity of isolation expertise in an commercial surroundings. it really is expected that Downstream Processing of Proteins: tools and Protocols will play a small half in filling this hole and therefore end up an invaluable contribution to the sphere. it's also designed to motivate academic strategists to develop the assurance of those issues in commercial biotechnology classes by means of together with debts of this crucial and swiftly constructing section of the commercial technique.

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Additional info for Downstream Processing of Proteins: Methods and Protocols (Methods in Biotechnology)

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2. Record the filtrate flow rate, water solution temperature, and the feed, retentate, and permeate pressures. 3. Calculate the permeability by dividing the filtrate flow rate by the mean transmembrane pressure drop and the membrane area. Values should be normalized to 25°C using a viscosity correction factor (typically supplied by the manufacturer). 4. Check the calculated value against the manufacturer’s specifications and any prior membrane lots or process runs. For the use of water permeability as a measure of the extent of fouling refer to Note 1.

Tech. 4, 329–334. 13. Baldwin, C. V. and Robinson, C. W. (1994) Enhanced disruption of Candida utilis using enzymatic pretreatment and high-pressure homogenization. Biotechnol. Bioeng. 43, 46–56. 14. Collis, M. A. and O’Neill, B. , and Middelberg, A. P. J. (1996) The effect of thermal deactivation on the properties and processing characteristics of Escherichia coli. Bioseparation 6, 55–63. 15. Watson, J. , Cumming, R. , and Tuffnell, J. M. (1987) Release of intracellular protein by thermolysis, in Separations for Biotechnology (Verrall, M.

4. Pumps Pumps supply both the tangential flow and required TMP. Although centrifugal pumps are inexpensive and deliver a smooth, nonpulsating flow, they are rarely used in bioprocessing becaise of potential particle formation by abrasion. Sliding-vane and gear pumps are also avoided because they can shed particles. The rotary lobe pump with two counterrotating impellers is used most frequently. Fluid is drawn around the outside of the impellers and squeezed out at the discharge port. The tight tolerances required for the impeller spacing make rotary lobe pumps fairly expensive.

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