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By Drogen, Tony Buehrer

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The homogenate was centrifuged as described above, and the pellet was homogenized three more times in the same way, with the supernatant retained after each centrifugation. /min using a Sorvall centrifuge for 45 min at 4oC. 13]. 2. ) foetal bovine serum, 1mM glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin in humidified air containing 5% CO2 at 37oC. 14]. 4 and 145mM NaCl (~108 cells). /min for 10 min at 4oC. The supernatant was reserved, the pellet was sonicated again and the mixture was centrifuged.

2. 8]. 9]. All reagents were prepared with Chelex 100 treated metal free water. The labelling reaction was allowed to proceed for 30 min at 80–100oC. Labelling procedures employing high activities were performed by reacting 100 and 200 μg of DOTATATE with 75 and 140 mCi of 177LuCl3, respectively, under the same reaction conditions. The effect of gentisic acid on preventing radiolytic effects was also investigated. The stability of these preparations was evaluated for 48 h. 2. 2 mm × 50 mm, 5 μm) with UV (230 nm) and radioactivity (Packard) detection.

The overall objective of the coordinated research project (CRP) on comparative evaluation of therapeutic radiopharmaceuticals was to develop reliable methodologies and evaluative capabilities to make prudent selections among therapeutic radiopharmaceuticals that can also be used for collection and submission of preclinical data. Specific objectives of the CRP included: — Development of methods for labelling, purification and quality control of therapeutic radiopharmaceuticals for neuroendocrine tumours based on different carrier molecules and radionuclides; — Standardization of in vitro methods for comparative evaluation of radiopharmaceuticals for biological integrity, cell binding, serum stability, kinetics, internalization and cytotoxicity; — Establishment of in vivo models for comparative evaluation of biodistribution, in vivo stability and therapeutic efficacy.

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