By P. G. Punchihewa (auth.), C. Oropeza, J. L. Verdeil, G. R. Ashburner, R. Cardeña, J. M. Santamaría (eds.)
The coconut palm occupies an important position on the planet financial system as an immense subsistence crop in all of the components during which it's grown. rather few international locations may be able to export any volume of coconut items due to expanding domestic calls for coupled with low productiveness. Yields are ordinarily good less than strength regardless of contemporary advancements with more desirable planting inventory and agronomic practices. within the final 50 years, either those facets have acquired huge awareness, however the concentration is transferring to enquire how using lately built biotechnological strategies- can gain the coconut undefined. This quantity, the results of the foreign Symposium on Coconut Biotechnology (held in December 1997 in Merida, Yucatan, Mexico), describes contemporary study in 3 vital parts. typical plant breeding concepts used with coconut have produced better planting fabric, yet development is necessarily very gradual. Can extra speedy genetic development be acquired utilizing molecular options? The papers provided during this part recommend that such strategies will open up intriguing new clients, yet purely after easy info has been accumulated at the genetic prestige of current coconut shares. examine utilizing microsatellite suggestions turns out to supply a useful gizmo to assist to classifying those shares. in spite of the fact that, just a blend of classical breeding tools with modem strategies will result in the swift development that is required to provide fabric for pressing replanting programs.
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Additional resources for Current Advances in Coconut Biotechnology
STMS has recently been applied to the characterisation of coconut germplasm (Karp, this volume). , this volume). This technique uses PCR to amplify the DNA in a known region of the genome which may then visualised using standard electrophoretic techniques. This technique has the advantage that allelic information may be derived but shares the large development cost of STMS. 36 Sequencing involves the determination of the base-pair sequences within the DNA of the plant. It is a very expensive and complex operation that may not reveal great polymorphism, but is highly reliable and gives co-dominant results (Table 1).
In coconut palms, AFLP has hitherto only been used to characterise genetic resources (Karp, this volume). STMS uses PCR to amplify microsatellite sequences, after which they are separated by electrophoresis to yield mUltiple co-dominant bands. Microsatellites are simple sequence repeats that are present in the genome in large copy numbers that vary greatly in length. The technique is able to detect a high level of polymorphism, is very reliable, fast and requires only slightly more skill than the simpler techniques.
2. Tagging o/specific traits The tagging of specific traits can be seen as a sub-set of genetic mapping that maps molecular markers to a specific trait. It forms the basis of marker-assisted selection (Paterson, 1996) and functions by finding molecular markers that are very closely linked to a gene or genes of interest. Marker-assisted selection would aid coconut improvement by making it possible to select for traits in the nursery, thus eliminating the need for costly and time-consuming field experiments.