By Prof. Dr. Germán Spangenberg, Dr. Zeng-Yu Wang, Prof. Dr. Ingo Potrykus (auth.)
This e-book exhibits the numerous development made in setting up the methodological foundation for the genetic manipulation of forage and turf grasses, with specific emphasis on our most crucial temperate grasses, the fescues and ryegrasses. It presents special and fantastically illustrated descriptions of all correct methodological points of molecular breeding of forage and turf grasses. the themes coated variety from the institution of plant regeneration structures from in vitro cultures, the restoration of haploids and somaclonal variations, the combo of entire or partial genomes through somatic hybridization, and the construction of transgenic vegetation, to the advance of molecular markers.
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Extra info for Biotechnology in Forage and Turf Grass Improvement
Tall fescue cultivars bred from Mediterranean ecotypes showed a lower regeneration frequency irrespective of the explants used for callus induction (Takamizo et al. 1994). Callus cultures of tall fescue have also proven valuable tools in studies relating to the tall fescue endophytic fungus Neotyphodium (formerly Acremonium) coenophialum. Conger and McDaniel (1983) have used 28-dayold callus cultures to screen tall fescue seed samples for the presence of the endophyte. This technique provided an alternative to the previously used histological procedures or enzyme-linked immunosorbent assays, which are more laborious or dependent on the examination of actively growing plants (Conger and McDaniel 1983).
J E. ~ (J 8 0 =t' ::; 0 ~. (I) ::; (I) fI<> 28 Callus Cultures and Somaclonal Variation vitro and required subculture at 20- to 25-day intervals. The cultures were maintained for over 2 years, retaining characteristics indicative of their endosperm origin, and did not show any morphogenic response. First callus cultures were initiated and established in F. arundinacea, F. pratensis, F. rubra and L. multifiorum from caryopses, isolated mature embryos, whole seedlings, primary root and leaf-tip explants from seedlings (Atkin and Barton 1973; Conger et al.
Plant Cell Rep 7: 644-647 Van der Valk P, Ruis F, Tettelaar-Schrier AM, Van de Velde CM (1995) Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass. The effect of benzyladenine. Plant Cell Tissue Organ Cult 40: 101-103 Vasil IK (1983) Regeneration of plants from single cells of cereals and grasses. In: Lurquin P, Kleinhofs A (eds) Genetic engineering of eukaryotes. Plenum Press, New York, pp 233-252 Vasil IK (1987) Developing cell and tissue culture systems for the improvement of cereals and grass crops.