By Professor Dr. med. Helmut Löffler, Professor Dr. med. Johann Rastetter (auth.)
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This e-book is an up to date evaluate of present wisdom within the box of a number of drug resistance in human melanoma. The literature as much as the center of 1993 is surveyed in professional chapters written by means of various specialists. themes coated comprise the molecular genetics, cytogenetics and biochemistry of the mdr genes and P-glycoprotein; replacement delivery proteins in MDR; topoisomerases I and II; cytochrome p450-enzymes and glutathione- S-transferases in MDR; mobile versions for MDR in good tumours and haemopoietic tumours; immunochemical and molecular organic options for detection of MDR-related gene expression; and medical and pharmacological innovations to bypass resistance.
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403). The highly motile orgamsms are clearly VlSIble even at low magnifIcation (250x). 2. Concentrating the sample: To 3-5 mL of drawn venous blood, add 10 - 15 mL of a mixture of 95 mL formalin (5 %), 5 mL acetic acid, and 2 mL of an alcoholic gentian violet solution (4 g per 100 mL 96 % alcohol). Centrifuge the mixture, and examine the sediment for stained micro filariae. ) 3. Examination of a skin snip for microfilariae (Onchocerca volvulus). Place a large drop ofphysiologic saline solution onto a slide.
Rhodesiense, T.. cruzi) . are stained in the same way as for malana parasItes. This method is also used to examine for Borrelia recurrentis. Bartonellosis Bartonella organisms are most readily detected by the examination of Pappenheim-, or Giemsa-stained blood smears. ow smears by Giemsa staining (17 mm) after fIxation in methanol (5 min) (see p. 7). 1 Toxoplasmosis Giemsa staining of the touch preparation or other sample is also recommended for the detection of toxoplasmosis. Direct immunofluorescence ~nd the peroxidase reaction can detect the orgamsm with high sensitivity.
The reticulocytes can be demonstrated by supravital staining (see p. 8). The myeloblasts, which are the precursors of neutrophilic granulocytes and monocytes, develop into neutrophilic promyelocytes and promonocytes. The eosinophilic and basophilic granulocytes pursue their own lines of development and therefore have their own promyelocytes with specific granules. Platelets (thrombocytes) develop from the cytoplasm of the megakaryocytes. The common progenitor cell from which monocytes and neutrophilic granulocytes originate might be termed the myelomonoblast (CFU-GM).